Characterization of Esterase A, a Pseudomonas stutzeri A15 Autotransporter
نویسندگان
چکیده
منابع مشابه
Characterization of Esterase A, a Pseudomonas stutzeri A15 Autotransporter.
Autotransporters are a widespread family of proteins, generally known as virulence factors produced by Gram-negative bacteria. In this study, the esterase A (EstA) autotransporter of the rice root-colonizing beneficial bacterium Pseudomonas stutzeri A15 was characterized. A multiple sequence alignment identified EstA as belonging to clade II of the GDSL esterase family. Autologous overexpressio...
متن کاملCharacterization of esterase A , an autotransporter of Pseudomonas stutzeri A 15 1 2
* Corresponding author. Centre of Microbial and Plant Genetics, K.U. Leuven, Kasteelpark 11 Arenberg 20 – Bus 2460, 3001 Heverlee, Belgium. Tel.: +32 16321631. Fax: +32 16321963. E12 mail: [email protected] 13 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.07690-11 AEM Accepts, published online ahead of print on 3 ...
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BACKGROUND Autotransporters represent a widespread family of secreted proteins in Gram-negative bacteria. Their seemingly easy secretion mechanism and modular structure make them interesting candidates for cell surface display of heterologous proteins. The most widely applied host organism for this purpose is Escherichia coli. Pseudomonas stutzeri A15 is an interesting candidate host for enviro...
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The search for new enzymes is facilitated by the rapidly growing number of genome sequences from different organisms. However, the discovery of functional proteins is still time intensive and complex alignments have to be performed. Herein, a genome database search identified a new, until now undescribed, putative lipase from Pseudomonas stutzeri strain A1501. The gene was cloned and expressed ...
متن کاملBiology of Pseudomonas stutzeri.
Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its part...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2012
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.07690-11